ZEISS Lightsheet 7


The light sheet fluorescence microscopy (LSFM) has become a standard method to image large optically cleared specimens in toto and with subcellular resolution. It splits fluorescence excitation and detection into two separate light paths, with the axis of illumination perpendicular to the detection axis. That means you can illuminate a single thin section of the sample at one time, generating an inherent optical section by exciting only fluorescence from the in-focus plane. No pinhole is required. Light from the in-focus plane is collected as the pixels of a camera, rather than pixel by pixel as, for example, in confocal or other laser scanning microscopes. Parallelization of the image collection on a camera-based detector lets you collect images faster and with less excitation light than you would with many other microscope techniques. Zeiss Lightsheet 7 is equipped with dedicated optics, sample chambers and sample holders to accurately adjust to the refractive index of all common clearing methods and immersion media to allow imaging of large samples, even whole mouse brains.

Detailed description

Detection optics:
Water immersion:
  • 5x / 0.16 foc (WD = 5.1 mm)
  • 10x / 0.5 foc (WD = 3.7 mm)
  • 20x / 1.0 foc (WD = 2.4 mm)
  • Fluar 2.5x / 0.12 M27 (WD = 8.7 mm)
  • 5x / 0.16 foc, n=1.33-1.58 (WD = 5.1 mm)
  • Clr Plan-Neofluar 20x / 1.0 Corr nd=1.45 (WD = 5.6 mm)
  • Clr Plan-Neofluar 20x / 1.0 Corr nd=1.53 (WD = 6.4 mm)
Illumination optics:
  • 5x / 0.1 foc
  • 10x / 0.2 foc

405 nm, 488 nm, 561 nm, 638 nm


  • Water chamber (n=1.33)
  • 20x Clearing chamber (n=1.35-1.58)
  • Large sample chamber (n=1.33-1.58)
  • Translucence chamber
Filters for fluorescence
DAPI-GFP, GFP-Cy3, GFP-mCherry, GFP-Cy5, Cy3-Cy5


2x PCO edge 4.2 sCMOS camera,1920 × 1920;
pixel size 6.5 μm × 6.5 μm


ZEN Black