ZEISS Elyra 7 with Lattice SIM
Description
The Elyra 7 is a motorized inverted microscope with resolution beyond the Abbe diffraction limit of the conventional light microscope. The microscope is equipped with a super-resolution system that uses a specialized lattice illumination pattern for 3D structured illumination (SIM) images and single-molecule localization methods (SMLM) like STORM and PALM. SIM is a technology that can be used with standard fluorophores, making it an easily accessible choice for super-resolution results. The lattice illumination allows for faster, gentler, and deeper imaging of live samples, and can reach up to 255 FPS during time-lapse acquisition. The unit has an incubation chamber for live imaging with controlled CO2 and temperature. For fast and precise Z-movement there is a piezo Z stage insert. The system yields up to 8-fold improvement in voxel resolution in the X, Y, and Z dimensions. The microscope is equipped with four solid-state lasers (405, 488, 561and 642 nm). The signal detection is assured by 2 sCMOS cameras which enable simultaneous detection of two different fluorophores. The high-tech offline PC workstation with high-capacity storage assures the fast processing of acquired samples.
Detailed description | |
Lasers | 405, 488, 561, 642 nm |
Objectives | For the current setting check table below. |
Filters for SIM and SMLM | detailed filter sets description |
Fluorescence light source | metal halide HXP 120 V |
Filters for fluorescence | F-set 77 HE – DAPI, GFP, mRFP, Alexa 633 |
Duolink | detailed duolink sets description |
Camera | 2 × PCO edge sCMOS camera, 1280 × 1280pixel size 6.5 μm × 6.5 μm |
Software | ZEN Black |
Current Elyra objectives and filters setup