ZEISS Elyra 7 s Lattice SIM

Description

The Elyra 7 is a motorized inverted microscope with resolution beyond the Abbe diffraction limit of the conventional light microscope. The microscope is equipped with a super-resolution system that uses a specialized lattice illumination pattern for 3D structured illumination (SIM) images and single-molecule localization methods (SMLM) like STORM and PALM. SIM is a technology that can be used with standard fluorophores, making it an easily accessible choice for super-resolution results. The lattice illumination allows for faster, gentler, and deeper imaging of live samples, and can reach up to 255 FPS during time-lapse acquisition. The unit has an incubation chamber for live imaging with controlled CO2 and temperature. For fast and precise Z-movement there is a piezo Z stage insert. The system yields up to 8-fold improvement in voxel resolution in the X, Y, and Z dimensions. The microscope is equipped with four solid-state lasers (405, 488, 561and 642 nm). The signal detection is assured by 2 sCMOS cameras which enable simultaneous detection of two different fluorophores. The high-tech offline PC workstation with high-capacity storage assures the fast processing of acquired samples.

Detailed description

Lasers

405, 488, 561, 642 nm
Lasers shared between Lattice SIM and SMLM
Objectives

For the current setting check table below.

Filters for SIM and SMLM
detailed filter sets description

Fluorescence light source

metal halide HXP 120 V

Filters for fluorescence

For the current setting check table below.

Duolinkdetailed duolink sets description

Camera

2 × PCO edge sCMOS camera, 1280 × 1280
pixel size 6.5 μm × 6.5 μm

Software

ZEN Black


Current Elyra objectives and filters setup