CORE FACILITY CELLULAR IMAGING
CEITEC Masaryk University
Kamenice 753/5
625 00 Brno CZ
cellim_support(at)ceitec.muni.cz
+420 54949 7085
+420 54949 6133
@Ceitec_CellimCF
Laser scanning confocal microscope Leica Stellaris 8 with Falcon FLIM
Description
The Leica STELLARIS 8 FALCON is a high-performance confocal microscope designed for advanced imaging and analysis. It features Fluorescence Lifetime Imaging (FLIM) with FALCON technology for real-time lifetime measurements, enabling studies of molecular interactions and environmental changes. The system incorporates LIGHTNING adaptive deconvolution to achieve super-resolution imaging. Its White Light Laser provides flexible excitation across the visible spectrum, and Power HyD detectors ensure high sensitivity and dynamic range. This system is optimized for multi-colour imaging, live-cell studies, and fixed sample analysis, offering precise and reproducible results for a wide range of applications.
Detailed description | |
Methods | Confocal Scanning Fluorescence Imaging: Equipped with the Lightning super-resolution module for enhanced imaging precision. Transmitted Light Imaging: Supports brightfield and Nomarski contrast (DIC), fully automated. Tile-Scanning & Image Merging: Enabled through LAS X software for seamless large-area imaging. Dynamic Experiments: Includes time-lapse and multi-position imaging capabilities. Photo-Kinetic Studies: Suitable for experiments like photobleaching (FRAP) and activation. FRET Analysis: Covers both Sensitized Emission (SE) and Acceptor Photobleaching methods. Advanced Techniques: Includes FLIM and FRET-FLIM for in-depth fluorescence lifetime and interaction studies. |
Lasers | 405 nm cw diode laser 488 nm cw FRAP laser 440 – 790 nm pulsed white light laser (WLL)
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| Detectors | 2x HyD S - most sensitive detector at 410 – 560 nm 2x HyD X - most sensitive detector at 560 – 740 nm. The fast detector is optimised for FLIM, and the image quality is better with a resonant scanner than HyD S. HyD R - most sensitive detector above 740 nm. The fast detector is optimised for FLIM, and the image quality is better with a resonant scanner than HyD S. T-PMT – transmitted light detector |
Transmitted light techniques | Brightfield and Nomarski contrast (DIC) (automated) |
Objectives | For the current setting check the table below |
Fluorescence light source | HXP 120W/45C VIS Hg lamp – Leica EL6000 |
Filters for fluorescence | DAPI (Ex. 355/56; DC: 400; Em. 460/50) DA/FI/TX (Ex. 403/18 + 493/18 + 575/25; DC: 435; 505; 595; Em. 456/30; 530/35; 630/60 GFP (Ex. 470/40; DC: 495; Em. 525/50) Y5 (Ex. 620/50; DC: 660; Em. 700/75 Analyser (for transmitted light DIC or POL with analyzer filter) |
Live cell imaging | OKOlab incubation system with integrated regulation of CO2, O2, temperature, and humidity |
Software | LAS-X Modules (fully implemented to the LAS X software):
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Current Leica Stellaris 8 Falcon objectives setup