
Evident IX83 IXplore SpinSR Yokogawa SoRa
Description
The IXplore SpinSR SoRa is a fully motorised spinning disk confocal microscope designed for high-speed, gentle live-cell imaging. Built on the Evident IX83 platform, it features a motorised objective turret, drift focus correction (DFC), and SoRa super-resolution technology, achieving ~120 nm lateral and ~300 nm axial resolution. With a microlens-enhanced dual Nipkow disk, sCMOS cameras, and 405–638 nm excitation, it supports 200 fps imaging, multimodal acquisition (widefield, confocal, super-resolution), and includes 405 and 488 nm FRAP lasers for photobleaching experiments. Integrated with cellSens, it enables precise multi-dimensional imaging, making it ideal for advanced cell biology and neuroscience research.
Methods
- Confocal spinning disk with 50um pinholes (standard confocal)
- Confocal spinning disk with SoRa technology (enhanced resolution up to 120 nm)
- Transmitted Light Imaging
- Tile-Scanning & Image Merging
- Dynamic Experiments: Includes time-lapse and multi-position imaging capabilities with HW autofocus
- Photo-Kinetic Studies: Suitable for experiments like photobleaching (FRAP) and activation
- Drift Focus Correction (DFC) system based on IR laser for real-time imaging and precise Z-axis stabilisation
Detailed description | |
Lasers | 405 nm; 445 nm; 488 nm; 515 nm; 561 nm; 638 nm 405 nm (100 mW) and 488 nm (150 mW) FRAP lasers |
Detectors | 2x Hamamtsu ORCA-Fusion BT sCMOS cameras (resolution 2034 x 2034 pixels, pixel size 6.5 x 6.5 µm, Quantum Efficiency [QE]: > 95 % at 550 nm |
Objectives | For the current setting, check the table below. |
Fluorescence light source | CoolLED pE-300white |
Filters for fluorescence | DA/FI/TX (Ex. 403/18 + 493/18 + 575/25; DC: 435; 505; 595; Em. 456/30; 530/35; 630/60) Y5 (Ex. 620/50; DC: 660; Em. 700/75) |
Live cell imaging | Pecon incubation unit including thermal chamber (CO2/O2, temperature, humidity) |
Transmitted light techniques | Brightfield |
Software | cellSens acquisition software |
Current IXploreSpinSR SoRa objectives setup