Evident IX83 IXplore SpinSR Yokogawa SoRa

Description

The IXplore SpinSR SoRa is a fully motorised spinning disk confocal microscope designed for high-speed, gentle live-cell imaging. Built on the Evident IX83 platform, it features a motorised objective turret, drift focus correction (DFC), and SoRa super-resolution technology, achieving ~120 nm lateral and ~300 nm axial resolution. With a microlens-enhanced dual Nipkow disk, sCMOS cameras, and 405–638 nm excitation, it supports 200 fps imaging, multimodal acquisition (widefield, confocal, super-resolution), and includes 405 and 488 nm FRAP lasers for photobleaching experiments. Integrated with cellSens, it enables precise multi-dimensional imaging, making it ideal for advanced cell biology and neuroscience research.

Methods

  • Confocal spinning disk with 50um pinholes (standard confocal)
  • Confocal spinning disk with SoRa technology (enhanced resolution up to 120 nm)
  • Transmitted Light Imaging
  • Tile-Scanning & Image Merging
  • Dynamic Experiments: Includes time-lapse and multi-position imaging capabilities with HW autofocus
  • Photo-Kinetic Studies: Suitable for experiments like photobleaching (FRAP) and activation
  • Drift Focus Correction (DFC) system based on IR laser for real-time imaging and precise Z-axis stabilisation

Detailed description

Lasers

405 nm; 445 nm; 488 nm; 515 nm; 561 nm; 638 nm

405 nm (100 mW) and 488 nm (150 mW) FRAP lasers

Detectors

2x Hamamtsu ORCA-Fusion BT sCMOS cameras (resolution 2034 x 2034 pixels, pixel size 6.5 x 6.5 µm, Quantum Efficiency [QE]: > 95 % at 550 nm

Objectives

For the current setting, check the table below.

Fluorescence light source

CoolLED pE-300white

Filters for fluorescence

DA/FI/TX (Ex. 403/18 + 493/18 + 575/25; DC: 435; 505; 595; Em. 456/30; 530/35; 630/60)

Y5 (Ex. 620/50; DC: 660; Em. 700/75)

Live cell imaging

Pecon incubation unit including thermal chamber (CO2/O2, temperature, humidity)

Transmitted light techniques

Brightfield

Software

cellSens acquisition software


Current IXploreSpinSR SoRa objectives setup