ZEISS Elyra 7 with Lattice SIM

COMING SOON

Description

The Elyra 7 is a motorized inverted microscope with resolution beyond the Abbe diffraction limit of the conventional light microscope. The microscope is equipped with a super-resolution system that uses a specialized lattice illumination pattern for 3D structured illumination (SIM) images and single-molecule localization methods (SMLM) like STORM and PALM. SIM is a technology that can be used with standard fluorophores, making it an easily accessible choice for super-resolution results. The lattice illumination allows for faster, gentler, and deeper imaging of live samples, and can reach up to 255 FPS during time-lapse acquisition. The unit has an incubation chamber for live imaging with controlled CO2 and temperature. For fast and precise Z-movement there is a piezo Z stage insert. The system yields up to 8-fold improvement in voxel resolution in the X, Y, and Z dimensions. The microscope is equipped with four solid-state lasers (405, 488, 561and 642 nm). The signal detection is assured by 2 sCMOS cameras which enable simultaneous detection of two different fluorophores. The high-tech offline PC workstation with high-capacity storage assures the fast processing of acquired samples.

Detailed description

Lasers

405, 488, 561, 642 nm
Lasers shared between Lattice SIM and SMLM

Objectives

Plan-Neofluoar 10x / 0.3 AIR,
C-Apochromat 40x / 1.2 W,
Plan-Apochromat 40x / 1.4 Oil,
Plan-Apochromat 63x / 1.46 OIL,
Plan-Apochromat 100x / 1.46 OIL,
Plan-Apochromat 100x / 1.57 OIL-HI

Filters for SIM and SMLM

Filter cube 1: SBS LP 560 with EF BP420-480/BP495-550 & EF BP570-620 & LP655

Filter cube 2: SBS SBS BP490-560/LP640 with EF BP420-480/BP570-620/LP740 & EF BP495-560/LP655

Fluorescence light source

metal halide HXP 120 V

Filters for fluorescence

F-set 77 HE – DAPI, GFP, mRFP, Alexa 633

Camera

PCO edge sCMOS camera, 1280 × 1280; pixel size 6.5 μm × 6.5 μm

Transmitted light techniques

Brightfield, Differential interference contrast, Phase contrast

Software

ZEN Black