CORE FACILITY CELLULAR IMAGING
CEITEC Masaryk University
Kamenice 753/5
625 00 Brno CZ
cellim_support(at)ceitec.muni.cz
+420 54949 7085
+420 54949 6133
@Ceitec_CellimCF
Laser scanning confocal microscope Zeiss LSM 780 with Airyscan detector
Description
Inverted microscope Zeiss Axio Observer.Z1 with laser scanning unit LSM 780. Microscope is equipped with three solid state lasers (405, 561 and 633 nm), argon laser (458, 488 and 514 nm), two multialkali PMT detectors, 32 channel spectral GaAsP detector, Airyscan detector and T-PMT for transmitted light detection. The Airyscan detector allows acquisition with increased resolution and signal to noise ratio compared to normal confocal scanning. Microscope is also equipped with CCD camera AxioCam MRm.
Detailed description | |
Lasers | 405, 458, 488, 514, 561, 633 nm |
Detectors | 2 x PMT detectors, 1 x GaAsp PMT spectral detector, 32-channels Airyscan detector, T-PMT detector |
| Filters for Airyscan | BP 420-480 + BP 495-550, BP 420-480 + BP 495-620, BP 420-480 + LP 605, BP 465-505 + LP 525, BP 495-550 + LP 570, BP 570-620 + LP 645 |
Objectives | For the current setting check table below. |
Fluorescence light source | HXP 120V |
Filters for fluorescence | DAPI (F46-000), CFP (F36-525), Cy3 (F36-542), Cy5 (F36-523) |
| Camera | Monochromatic camera Axiocam MRm, CCD sensor, 1388 x 1040 pixels, pixel size 6.45 x 6.45 μm |
Transmitted light techniques | Brightfield, Differential interference contrast, Phase contrast |
Software | ZEN Black |
Current LSM 780 objectives setup