CORE FACILITY CELLULAR IMAGING
CEITEC Masaryk University
Kamenice 753/5
625 00 Brno CZ
cellim_support(at)ceitec.muni.cz
+420 54949 7085
+420 54949 6133
@Ceitec_CellimCF
Laser scanning confocal microscope Zeiss LSM 800
Description
Inverted microscope Zeiss Axio Observer.Z1 with confocal unit LSM 800 and incubation chamber for live imaging with controlled CO2 and temperature. Microscope is equipped with four solid state lasers, three GaAsP PMT detectors and one T-PMT detector for transmission light detection. Widefield imaging is possible using Hamamatsu ORCA-Flash 4.0LT digital sCMOS camera. Microscope is equipped with Definite Focus.2 to keep sample in focus during long time-lapse experiments.
Detailed description | |
Lasers | 405, 488, 561, 640 nm |
Detectors | 3 x GaAsp PMT, 1 x T- PMT detector |
Objectives | For the current setting check table below. |
Fluorescence light source | HXP 120V |
Filters for fluorescence | DAPI (F-set 49), GFP (F-set 38He), Cy3 (F-set 63He), Cy5 (F-set 50) |
| Transmitted light techniques | Brightfield, Differential interference contrast, Phase contrast |
Camera | Monochromatic camera ORCA-Flash 4.0LT digital sCMOS, 2084 x 2084 pixels, 6.5 x 6.5 μm size |
Software | ZEN Blue |
Current LSM800 objectives setup