Laser scanning confocal microscope Zeiss LSM 880 with AiryscanFast module

Description

Inverted microscope Zeiss Axio Observer.7 with laser scanning unit LSM 880 and incubation chamber for live imaging with controlled CO2 and temperature. Microscope is equipped with four solid state lasers (405, 561, 594 and 633 nm), argon laser (458, 488 and 514 nm), two multialkali PMT detectors, 32 channel spectral GaAsP detector, Airyscan detector and T-PMT for transmitted light detection. The Airyscan detector allows acquisition with increased resolution and signal to noise ratio compared to normal confocal scanning. In fast mode the detector is capable of 4x faster acquisition compare to standard mode. For fast and precise Z-movement there is a piezo Z stage insert - available on demand.


Detailed description

Lasers

405, 458, 488, 514, 561, 594, 633 nm

Detectors

2 x PMT detectors, 1 x GaAsp PMT spectral detector, AiryscanFast detector, T-PMT detector

Objectives

For the current setting check table below.

Filters for Airyscan

BP420-480 + LP 605, BP465-505 + LP 525, BP495-550 + LP 570, BP555-620 + LP 645, BP420-480 + BP 495-550, BP420-445 + BP 465-505

Fluorescence light source

HXP 120V

Filters for fluorescence

DAPI (F-set 49), CFP (F-set 47), GFP (F-set 38), Cy3 (F-set 63), Cy5 (F-set 50)
CameraMonochromatic camera Axiocam MRm, CCD sensor, 1388 x 1040 pixels, pixel size 6.45 x 6.45 μm

Transmitted light techniques

Brightfield, Differential interference contrast

Software

ZEN Black


Current LSM880 objectives setup